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Fluorescence Measurement Modes

CD Spectrometer - J-815 Series

The J-815 offers two optional methods for measurement of Fluorescence. Each of these methods allows either simultaneous or separate measurement of both CD and Fluorescence on the same sample.

The simplest method employs the TFA-335 Total Fluorescence option utilizing high pass cut-off filters for Emission wavelength selection. This low cost method allows simple yet sensitive detection of Fluorescence changes during a titration or thermal ramp and also allows spectral Excitation scans.

 

FMO-427

The FMO-427 utilizes a high efficiency monochromator for Emission wavelength selection.  In addition to Excitation scans, the FMO-427 allows Emission scans to follow changes in Emission wavelength during an experiment.

 

Both the TFA-335 and FMO-427 are used in conjunction with either the PFD-425 or MPTC-490 Peltier temperature control systems.

 

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